quantitect reverse transcription kit (Qiagen)

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Qiagen quantitect reverse transcription kit
Quantitect Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 18973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitect+probe+pcr+kit/Reverse+Transcription+QuantiTect+Probe+RT-PCR+Kit/pm42123637-312-16-20
Average 99 stars, based on 18973 article reviews

quantitect reverse transcription kit - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Temperature-dependent survival of Mycoplasma anserisalpingitidis in water: implications for biosecurity and transmission in waterfowl farming.
Article Snippet: .. The reaction was performed using a QuantiTect Probe PCR Kit (Qiagen) with a total volume of 25 μL, consisting of 12.5 μL of master mix, 1.3 μL of each 10 μM primer, 0.5 μL of probe, 7.4 μL of distilled water, and 2 μL of DNA. .. Amplification was carried out in an ABI 7500 RealTime PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) with initial denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 58 °C for 1 min.

Article Title: Serologic and molecular prevalence of Anaplasma marginale in Missouri beef herds.
Article Snippet: Anaplasma marginale qPCR DNA was extracted from 200 μL of EDTAanticoagulated whole blood using the GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions, with a final elution volume of 200 μL. .. Samples were tested for A marginale using primers and a probe designed to amplify the msp1b gene.34 Each reaction consisted of 10 μL of master mix (QuantiTect Probe PCR Kit; Qiagen), 1 μL each of A marginale forward and reverse primers and 1 μL of A marginale probe (Integrated DNA Technologies), 1 μL of 25 mM magnesium chloride, 1 μL of molecular biology–grade water, and 5 μL of DNA template in a 20-μL total reaction volume. ..

Article Title: Development and clinical evaluation of a novel SHERLOCK test for Mycoplasma genitalium
Article Snippet: .. The fluorescent dye of the probe was changed from Cy5 to FAM ( ). qPCR was conducted using a QuantiTect Probe PCR Kit (QIAGEN). .. The final reaction volume was 20 μL, with 2 μL DNA template. qPCR was carried out on a QuantStudio 5 (Applied Biosystems, Waltham, MA), with the following thermal conditions: initial activation at 95°C for 15 min, followed by 45 cycles of denaturation at 94°C for 15 s and annealing/extension at 60°C for 1 min. Ten-fold serial dilutions of AMPLIRUN MYCOPLASMA GENITALIUM DNA CONTROL (Vircell S.L., Granada, Spain), ranging from 1.0 to 10,000 copies/reaction, were used to estimate the MG DNA copy number in the specimens.

Article Title: Decreased Glucocorticoid Receptor Expression and Function in Cord Blood Immune Cells from Preterm Neonates with Morbidity.
Article Snippet: .. GR isoform mRNA was measured using Taqman probe qPCR with a QuantiTect Probe PCR kit (Qiagen, Germantown, MD, USA, #204343) and various PrimeTime double-quenched probes (IDT), containing a 5′FAM fluorphore, internal ZEN quencher, and 3′ Iowa Black FQ quencher for enhanced specificity and reduced background signal. .. The Bio-Rad iCycler equipped with real-time optical fluorescent detection system was used for SYBR Green and FAM detection.

Article Title: Temperature-dependent survival of Mycoplasma anserisalpingitidis in water: implications for biosecurity and transmission in waterfowl farming
Article Snippet: .. The reaction was performed using a QuantiTect Probe PCR Kit (Qiagen) with a total volume of 25 μL, consisting of 12.5 μL of master mix, 1.3 μL of each 10 μM primer, 0.5 μL of probe, 7.4 μL of distilled water, and 2 μL of DNA. .. Amplification was carried out in an ABI 7500 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) with initial denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 58 °C for 1 min.

Article Title: Association between LDL-R (exon 8 C.1171 G > A) polymorphisms and response to antiviral therapy in hepatitis C virus infection
Article Snippet: .. Genotyping of LDL-R allelic variations was conducted using real-time PCR with the QuantiTect ® Probe PCR Kit (Qiagen, Hilden, Germany) on a StepOne Real-Time PCR system. ..

Article Title: Decreased Glucocorticoid Receptor Expression and Function in Cord Blood Immune Cells from Preterm Neonates with Morbidity
Article Snippet: .. GR isoform mRNA was measured using Taqman probe qPCR with a QuantiTect Probe PCR kit (Qiagen, Germantown, MD, USA, #204343) and various PrimeTime double-quenched probes (IDT), containing a 5′FAM fluorphore, internal ZEN quencher, and 3′ Iowa Black FQ quencher for enhanced specificity and reduced background signal. .. The Bio-Rad iCycler equipped with real-time optical fluorescent detection system was used for SYBR Green and FAM detection.

Article Title: First evidence of recombinant Myxomavirus (ha-MYXV) in European hare ( Lepus europaeus ) in the Czech Republic and Slovakia
Article Snippet: Total nucleic acid was extracted from 200 μl of organ pool supernatant by MagNA Pure 24 (Total NA Isolation Kit, Roche) and eluted into 50 μl. .. The ha-MYXV was detected and typed by a multiplex qPCR assay (QuantiTect Probe PCR kit, Qiagen) for detection and differentiation between the classical and ha-MYXV variants ( ). ..

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